sufS [2017-11-10 12:52:00]

cysteine desulfurase, cysteine:SufU sulfuryl transferase

locus: BSU32690

pI: 5.20

mw: 44.76 kDa

protein length: 406 aa

gene length: 1218 bp

function: formation of iron-sulfur clusters in proteins

product: cysteine desulfurase

essential: yes

ec: 2.8.1.7

synonyms: yurW, csd

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.3|Biosynthesis of iron-sulfur clusters]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,357,444 → 3,358,664

Phenotypes of a mutant

• essential [Pubmed|12682299]
• terminal phenotype of a CRISPRi knockdown mutant is moderate bulging [Pubmed|27238023]

The protein

Catalyzed reaction/ biological activity

• transfer of iron to [protein|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|SufU] [Pubmed|21236255]

Protein family

• Csd subfamily (according to Swiss-Prot)

Paralogous protein(s)

• [protein|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|NifS], [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|YrvO], [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|NifZ], [protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|YcbU]

[SW|Cofactors]

• pyridoxalphosphate [Pubmed|25447671]

Effectors of protein activity

• interaction with [protein|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|SufU] enhances the activity of [protein|88ACE6B79534338F7C72F107B35A0B2384007088|SufS] 40-fold [Pubmed|20097860]

Structure

• [PDB|5J8Q] [Pubmed|27382962]

Expression and Regulation

Operons

• genes: [gene|E966F3808222820C0644DC98F95765B89E6A2551|sufC]-[gene|1A9DA839AB77AF53958CB8426607BEF14F62B85E|sufD]-[gene|88ACE6B79534338F7C72F107B35A0B2384007088|sufS]-[gene|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|sufU]-[gene|5991F051C5BAD61A0860BBC64A37DB9D16130998|sufB]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|22383849], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-B585 (yurW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1584 NBRP B. subtilis, Japan]
• BEC32690, a CRISPRi knockdown mutant of ''[gene|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|sufU]'', is available from the [http://bgsc.org/ Bacillus Genetic Stock Center]

References

Reviews

• 25447671

Original publications

• 21236255,20097860,20822158,22383849,24321018,15379559,27382962,27238023